Recombinant Mouse Acidic Fibroblast Growth Factor (Mouse aFGF)
产品参数
|
别名（Synonyms） |
Fibroblast growth factor 1；FGF-1；Acidic fibroblast growth factor；HBGF-1 |
|
Accession |
P61148 |
|
GeneID |
14164 |
|
表达系统（Source） |
E.coli-derived mouse Acidic Fibroblast Growth Factor protein, Phe16-Asp155 |
|
分子量大小（Molecular Weight） |
Approximately 15.8 kDa. |
|
氨基酸序列(AA Sequence) |
FNLPLGNYKK PKLLYCSNGG HFLRILPDGT VDGTRDRSDQ HIQLQLSAES AGEVYIKGTE TGQYLAMDTE GLLYGSQTPN EECLFLERLE ENHYNTYTSK KHAEKNWFVG LKKNGSCKRG PRTHYGQKAI LFLPLPVSSD |
|
标签（Tag） |
None |
|
外观（Physical Appearance） |
Sterile Filtered White lyophilized (freeze-dried) powder. |
|
纯度（Purity） |
> 96 % by SDS-PAGE and HPLC analyses. |
|
生物活性（Biological Activity） |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg in the presence of 10 μg/ml of heparin. |
|
内毒素（Endotoxin） |
< 1.0 EU per 1μg of the protein by the LAL method. |
|
制剂（Formulation） |
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4. |
|
复溶（Reconstitution） |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
储存条件
产品用冰袋装运，可在-20℃至-80℃下保存1年。
建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。
注意事项
1.避免反复冻融循环。
2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。
3.仅供研究使用！
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