Recombinant Mouse RANKL/sRANK Ligand/TNFSF11 Protein

别名

soluble Receptor Activator of NF-κB Ligand, TNFSF11, OPGL, ODFTRANCE (TNF-related activation-induced cytokine)

种属

Mouse

表达序列

Pro143-Asp316

Accession

O35235

标签

No Tag

表达系统

E.coli

分子量大小

Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.

纯度

> 96% as determined by SDS-PAGE.

内毒素

< 0.01 EU per μg by the LAL method.

制剂

Lyophilized from a 0.2µm filtered concentrated solution in 20mMPB, 300mM NaCl, pH7.4.

外观

Lyophilized Powder.

生物活性

Fully biologically active when compared to standard. Determined by its dose-dependent ability to induce reporter gene in HT-29 NF-κB Luc reporter cells, The ED50 for this effect is 0.5-2 ng/ml.

复溶方法

Before use this product, please read the direction below carefully.1.This vial must be briefly centrifuged prior to opening to bring the contents to the bottom.2.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml.Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.