Recombinant Mouse soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A (Mouse sTNF RI/TN

产品参数

别名(Synonyms)

CD120a; FPF; p55

Accession

P25118

GeneID

21937

表达系统(Source)

E.coli-derived Mouse sTNF RI/TNFRSF1A, Ile22-Ala212.

分子量大小(Molecular Weight)

Approximately 21.1 kDa.

氨基酸序列(AA Sequence)

IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A

标签(Tag)

None

外观(Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

纯度(Purity)

> 97 % by SDS-PAGE and HPLC analyses.

生物活性(Biological Activity)

The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1 μg/mL, corresponding to a specific activity of > 1000 IU/mg in the presence of 0.1 ng/mL of rMuTNF-α. Fully biologically active when compared to standard. 

内毒素(Endotoxin)

< 0.1 EU per 1μg of the protein by the LAL method.

制剂(Formulation)

Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH7.4.

复溶(Reconstitution)

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in appropriate buffered solutions.

 

储存条件

产品用冰袋装运,可在-20℃至-80℃下保存1年。

建议第一次使用时将蛋白质分成较小的量,并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康,请穿实验服,戴一次性手套进行操作。

3.仅供研究使用!

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