Mouse IL-36γ, 152aa

产品描述

IL-36 gamma [previously called IL-1F9, IL-1 epsilon (epsilon), and IL-1H1] is a member of the IL-1 family which includes IL-1 beta, IL-1 alpha, IL-1ra, IL-18, IL-36 Ra (IL-1F5), IL-36 alpha (IL-1F6), IL-36 beta (IL-1F8), IL-37 (IL-1F7) and IL-1F10. All family members show a 12 beta-strand, beta-trefoil configuration, and are believed to have arisen from a common ancestral gene. Mouse IL-36 gamma is an 18-22 kDa, 164 amino acid (aa) intracellular and secreted protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation sites. Mouse IL-36 gamma (aa 13-164) shares 58%, 84%, 64% and 60% aa sequence identity with human, rat, equine and bovine IL-36 gamma, respectively, and 23-57% aa sequence identity with other family members. A 193 aa mouse isoform with a 29 aa N-terminal extension has been reported. Highest levels of IL-36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to first-line defense against pathogens in the skin, lungs and digestive tract. Its expression is induced by LPS treatment of monocytes, and by IL-alpha / beta, IL-17 or TNF-alpha treatment of keratinocytes and bronchial epithelia. Skin IL-36 gamma expression is increased in contact hypersensitivity and psoriasis. It is elevated in inflammatory disorders of the lung (such as asthma) and viral infections. Lung IL-36 gamma and other IL-36 proteins contribute to neutrophil influx. The receptor for IL-36 gamma is a combination of IL-1 Rrp2, mainly found in epithelia and keratinocytes, and the widely expressed IL-1 RAcP. IL-36 alpha, beta and gamma all activate NF-kappa B and MAPK pathways in an IL-1 Rrp2 dependent manner, and IL-36 gamma induces production of inflammatory cytokines and chemokines such as CXCL8/IL-8. Full-length recombinant IL-36 proteins appear less active than their endogenous counterparts, but trimming of the N-termini enhances their activity.

 

产品参数

别名（Synonyms）	IL1F9; IL-1F9; IL1H1; IL-1H1
Accession	Q8R460
GeneID	Mm.249379.
表达系统（Source）	E.coli-derived Mouse IL-36γ, 152aa, Gly13-Ser164.
分子量大小（Molecular Weight）	Approximately 17.3 kDa.
氨基酸序列(AA Sequence)	GRETPDFGEV FDLDQQVWIF RNQALVTVPR SHRVTPVSVT ILPCKYPESL EQDKGIAIYL GIQNPDKCLF CKEVNGHPTL LLKEEKILDL YHHPEPMKPF LFYHTRTGGT STFESVAFPG HYIASSKTGN PIFLTSKKGE YYNINFNLDI KS
标签（Tag）	None
外观（Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 97% by SDS-PAGE and HPLC analyses.
生物活性（Biological Activity）	The ED50 as determined by inducing IL-6 secretion in murine NIH/3T3 cells is less than 10 ng/mL, corresponding to a specific activity of > 1.0 × 105 IU/mg. Fully biologically active when compared to standard.
内毒素（Endotoxin）	< 0.1 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from a 0.2 µm filtered solution in 1 M MOPS, 10 mM NaAC, pH7.6, with 2 mM EDTA, 5% Trehalose, 0.02 % Tween-20.
复溶（Reconstitution）	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

储存条件

产品用冰袋装运，可在-20℃至-80℃下保存1年。

建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。

3.仅供研究使用！