Mouse IL-36RA

产品描述

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1δ [delta], IL1HY1, IL-1H3, and IL-1L1) is a member of the IL1 family of proteins. IL1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL18 and IL1F5 through F10. All family members show a 12 beta -strand, beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications. The mouse IL36Ra/IL1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL18), supporting an evolutionary relationship with the IL-1 family. It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. In humans, there is an alternate start site that potentially gives rise to an alternate splice form. This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL36Ra/IL1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL1ra, IL1 beta, IL36 alpha /IL1F6, IL37/IL1F7, IL-36 beta /IL-1F8, IL36 gamma /IL1F9 and IL1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells. The receptor for IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL1 Rrp2 and/or IL-1 RAcP. In either case, activity association with receptor binding is unclear. It was initially reported to be an antagonist of  IL-36 gamma activity. This would be consistent with its hypothesized relationship to IL1RA.

 

产品参数

别名（Synonyms）	IL-1HY1, IL-1 delta, IL-1F5, IL-1H3, IL-1L1
Accession	Q9QYY1
GeneID	54450
表达系统（Source）	E.coli-derived Mouse IL-36RA, Val3-Asp156.
分子量大小（Molecular Weight）	Approximately 16.9 kDa.
氨基酸序列(AA Sequence)	VLSGALCFRM KDSALKVLYL HNNQLLAGGL HAEKVIKGEE ISVVPNRALD ASLSPVILGV QGGSQCLSCG TEKGPILKLE PVNIMELYLG AKESKSFTFY RRDMGLTSSF ESAAYPGWFL CTSPEADQPV RLTQIPEDPA WDAPITDFYF QQCD
标签（Tag）	None
外观（Physical Appearance）	Sterile Filtered White lyophilized (freeze-dried) powder.
纯度（Purity）	> 95% by SDS-PAGE and HPLC analyses.
生物活性（Biological Activity）	Measured by its ability to inhibit IL-36γ induced IL-6 production by human PBMCs.
内毒素（Endotoxin）	< 1.0 EU per 1μg of the protein by the LAL method.
制剂（Formulation）	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
复溶（Reconstitution）	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.

 

储存条件

产品用冰袋装运，可在-20℃至-80℃下保存1年。

建议第一次使用时将蛋白质分成较小的量，并避免反复冻融循环。

 

注意事项

1.避免反复冻融循环。

2.为了您的安全和健康，请穿实验服，戴一次性手套进行操作。

3.仅供研究使用！